Magnetic nanoparticles for specifically capturing endothelial progenitor cells and evaluation of its cellular compatibility
1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, P.R.China
2. Key Laboratory of Advanced Technologies of Materials (KLATM), Ministry of Education, Southwest Jiaotong University, Chengdu 610031, P.R.China
Immobilization of CD34 antibody on ferroferric oxide magnetic nanoparticles was achieved by the traditional carboxyl-amine conjugation reaction. Fourier transform infrared spectroscopy (FT-IR), nanoparticle size analysis (dynamic light scattering and transmission electron microscope), and other testing methods were used to detect the surface modified magnetic nanoparticles. The endothelial progenitor cells (EPCs) were cultured with the surface modified magnetic nanoparticles to evaluate cell compatibility and the combination effect of nanoparticles on EPCs in a short period of time. Directional guide of the surface modified magnetic nanoparticles to EPCs was evaluated under applied magnetic field and simulated dynamic flow condition. The results showed that the magnetic nanoparticles were successfully modified with CD34 antibody, which had good cell compatibility within a certain range of the nanoparticle concentrations. The surface modified nanoparticles can combine with EPCs effectively in a short time, and those nanoparticles combined EPCs can be directional guided under the magnetic field in the dynamic flow environment.
不同浓度 Fe3O4-PEG@CD34 纳米粒子与 EPCs 共同培 养的 CCK-8 结果（*P < 0.05，** P < 0.01）
The CCK-8 results of EPCs incubated with different concentrations of the magnetic nanoparticles (*P < 0.05, ** P < 0.01)
动态磁场作用下，Fe3O4-PEG 和 Fe3O4-PEG@CD34 在 不同时间内对 EPCs 的捕获计数（**P < 0.01，*** P < 0.001）
The number of captured EPCs in different times by Fe3O4-PEG and Fe3O4-PEG@CD34 magnetic nanoparticles in dynamic magnetic field (**P < 0.01, *** P < 0.001)
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